Cell Membrane Mimicking Models

We have focussed on constructing bacterial-membrane mimicking systems including Gram-positive membranes, Gram-negative inner and outer-membranes for studying the transport of small hydrophilic molecules across membranes, membrane proteins and understanding pore-forming properties of antimicrobial peptides.  We employ Giant unilamellar vesicles (GUVs) as our membrane model system. Apart from working on membrane transport, we have also investigated the role of native lipids in membrane bending and shape transformation employing the engineered cell membrane mimicking models. 

Engineering vesicles with defined lipid composition mimicking bacterial membrane 

Gram-positive membrane model

Gram-negative membrane model

gram-negative mimicking membrane, LPS containing vesicles, Giant unilamellar vesicles containing LPS

Inner-membrane (IM)

Outer-membrane (GN)

Membrane proteins

Antimicrobial peptides

membrane proteins and antimicrobial peptide, antibiotic transport assay, antimicrobial peptide interaction with LPS membrane

Native bacterial lipids triggered shape changes in vesicle membranes

Shape changes occurring in the vesicles are caused by their mechanistic and intrinsic characteristics like membrane rigidity and curvature due to respective lipid composition and arrangement

Fluctuation of membranes observed in two systems- IM vesicle and OM vesicle (with LPS)

OM/LPS Vesicle after deflation assuming nanotube connecting daughter vesicle structure